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Stools are collected in the acute stage of the illness in a clean bowl, hence transferred into a sterile pot using a spatula, then sent to the laboratory. Samples can be stored in the fridge (4°), for a maximum of 24 hours.
Nappies are difficult to manipulate and cause false negatives.
If there is a suspicion of Shigella, prompt culturing and careful handling of samples is necessary since Shigella has limited ability to survive outside the human body
If testing for a parasite, always use a non-absorbent sample packaging (no paper, cardboard, cloth, etc.). One negative test is not sufficient; the test must be repeated at least 3 times in 3 or 4-day intervals.
Sampling is usually repeated for 3 consecutive days. The patient can bring the 3 samples on the 3rd day.
Stool testing for blood should not be sampled during menstruation.
Enterobius vermicularis
For the identification of Enterobius vermicularis (threadworm/pin worm) material should be obtained first thing in the morning on awakening by using a clear adhesive tape slide. Place the sticky side of a strip of tape over the anal region to obtain the material and stick the tape onto a glass slide.